The Effect Of Ph On Enzymatic Reaction

Title : Factor Affecting Enzyme Activity (pH) Objectives 1. To read the following(a) instruction manual carefully. 2. To determine the significant effects of different pH levels on enzyme activities. 3. Find out the optimal pH execute for the reactions of the attached enzyme through a plan graph. Variables Independent variables : pH of resoluteness in streak tube Dependent variables : Time for slice of colour of the dissolvent Constant variables : Total volume of solution in distributively test tube, volume of stiffen in apiece test tube, temperature of the solution. Materials Beakers, test tubes in a rack, dropper, bell ringer pen, pipettes, sucker, global indicator paper, stop watch, distilled water, fresh starch solution, one solution, amylase, 0,1mol/L hydrochloric astringent (pH=1), 0.1mol/L acetic bitter (pH=3), 0.001mol/L sodium hydrated oxide (pH=11), 0.1 mol/L sodium hydroxide (pH=13), trumpery rod, grooved white tile. Pr ocedures 1. The pH of both dot and two basic was recorded in the table below. 2. 5 test tubes with the solution were set up as shown as in the table below. 3. The contents of each test tubes were conflate and the pH was checked. It was written down to the right of the first in the pH column if the pH is different from the pH recorded. 4.

alone the tubes were find ad the time when the blue colour disappeared in each tube was noted. 5. The reciprocal of each time was metrical and the harbor was inserted in the 1/time column. This was used as the exercise of the enzyme. 6. A graph o f activity (1/time) against pH was plotted o! n a graph paper. Results Test tubes |mL starch |mL iodine |mL amylase |mL distilled water |mL of acid or basic |pH |Time for fade of colours, t (s) |1/time (s-1) | | | | | | | |Before |After | | | |1 |3 |3 |3 |1 |0 |7 |6 |cl |0.0067 | |2 |3 |3 |3 |0 |1 mL of 0.1 mol/L HCL |1 |3 |240 |0.0042 | |3 |3 |3 |3 |0 |1 mL of 0.1 mol/L acetic acid |3 |5 |195 |0.0051 | |4 |3 |3 |3 |0 |1 mL of 0.001...If you want to get a full essay, put up it on our website: OrderCustomPaper.com

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